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ldn 193189 stemgent  (ReproCELL)

 
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    ReproCELL ldn 193189 stemgent
    Ldn 193189 Stemgent, supplied by ReproCELL, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ldn+193189+stemgent/pm39255062-729-8-9
    Average 95 stars, based on 1 article reviews
    ldn 193189 stemgent - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFAP DAKO Cat# N1506; RRID: AB_10013482 Mouse monoclonal anti-S100b Sigma-Aldrich Cat# S2532; RRID: AB_477499 Rabbit polyclonal anti-OLIG2 EMD Millipore Cat# AB9610; RRID: AB_570666 Mouse monoclonal IgM anti-O4 Sigma-Aldrich Cat# O7139; RRID: AB_477662 Anti-O4 MicroBeads Miltenyi Biotec Cat# 130-096-670; RRID: AB_2847907 Goat polyclonal anti-SOX10 R&D systems Cat# AF2864; RRID: AB_442208 Rat monoclonal anti-MBP Millipore Cat# MAB386; RRID: AB_94975 Rabbit polyclonal anti-Cleaved Caspase-3 Cell Signaling Technology Cat# 9661; RRID: AB_2341188 Rabbit polyclonal anti-STAT1 Santa Cruz Cat# sc-346; RRID: AB_632435 Rabbit monoclonal anti-Phospho-STAT1 Cell Signaling Technology Cat# 9167; RRID: AB_561284 Rabbit monoclonal anti-CLUSTERIN Cell Signaling Technology Cat# 34642; RRID: AB_2799057 Mouse monoclonal anti-CLUSTERIN Santa Cruz Cat# sc-5289; RRID: AB_673566 Mouse monoclonal anti-CXCL10 R&D systems Cat# MAB266; RRID: AB_2261309 Rabbit polyclonal anti-TDP-43 Proteintech Cat# 10782-2-AP; RRID: AB_615042 Goat polyclonal anti- Galectin-7 R&D systems Cat# AF1339; RRID: AB_2297076 Mouse monoclonal anti-hnRNP E2 Santa Cruz Cat# sc-101136; RRID: AB_1124684 Rabbit polyclonal anti- Neurofilament 200 Sigma-Aldrich Cat# N4142; RRID: AB_477272 Goat polyclonal anti-SOX9 R&D systems Cat# AF3075; RRID: AB_2194160 Biological samples Human brain tissue Banner Sun Health Research Institute www.brainandbodydonationprogram.org Chemicals, peptides, and recombinant proteins DMEM/F12 GIBCO Cat# 11330-032 Matrigel Corning Cat# CB40230 mTeSR1 Stem Cell Technologies Cat# 85850 Y-27632 Reprocell Cat# 04-0012-10 Accutase Sigma-Aldrich Cat# A6964 N2 Life Technologies Cat# 17502048 B27 Life Technologies Cat# 12587010 GlutaMax GIBCO Cat# 35050079 NEAA Thermo Fisher Scientific Cat# 11140076 CHIR99021 Cellagen Technology Cat# C2477-50 SB431542 Stemgent Cat# 04-0010 LDN-193189 Stemgent Cat# 04-0074 Smoothened agonist (SAG) EMD Millipore Cat# 566660 bFGF PeproTech Cat# 100-18B EGF PeproTech Cat# 100-15 Retinoic acid Sigma-Aldrich Cat# R2625 CNTF R&D systems Cat# 257-NT-050 PDGFAA R&D systems Cat# 221-AA-050 IGF-1 R&D systems Cat# 291-G1-200 HGF R&D systems Cat# 294-HG-025 NT3 EMD Millipore Cat# GF031 3,30,5-Triiodo-L-thyronine (T3) Sigma-Aldrich Cat# T2877 Biotin Sigma-Aldrich Cat# 4639 (Continued on next page) 20 Cell Reports 42, 112841, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 L-Ascorbic acid Sigma-Aldrich Cat# A4403 Inuslin Sigma-Aldrich Cat# I9278 TGFa Stemcell Techology Cat# 78157.1 IL1b R&D systems Cat# 201-LB-005 Human Clusterin Protein R&D Systems Cat# 2937-HS-050 Critical commercial assays Tetro cDNA Synthesis kit BioLINE Cat# Bio-65043 SYBR Green Master Mix Thermo Scientific Cat# F416L P3 4D nucleofection kit Lonza Cat# V4XP-3024 Human Clusterin Quantikine ELISA Kit R&D Systems Cat# DCLU00 HUMAN IP-10/CXCL10 ELISA KIT MilliporeSigma Cat# RAB0119 Click-iTTM Plus EdU Cell Proliferation Kit Invitrogen Cat# C10637 Magna ChIPTM A/G Chromatin Immunoprecipitation Kit Sigma Cat# 17-10085 CUT&RUN Assay Kit Cell Signaling Technology Cat# 86652 Dual-Luciferase Reporter Assay System Promega Cat# E1910 Alexa FluorTM 488 Tyramide SuperBoostTM Kit, goat anti-mouse IgG Invitrogen Cat# B40912 hPSC Genetic Analysis Kit Stemcell Techology Cat# 07550 PureLinkTM Genomic DNA Mini Kit Invitrogen Cat# K182001 Deposited data Mass spectrometry analysis of differential binding to the C vs. T allele by nuclear proteins This paper MassIVE: MSV000088660 RNA-seq analysis of C/C vs. T/T or C/T astrocytes This paper GEO: GSE193218 Experimental models: Cell lines ADRC18 fibroblast UCI-ADRC N/A AG14048 fibroblasts Coriell Cat# AG14048 AG06869 fibroblasts Coriell Cat# AG06869 Human primary astrocytes ScienCell Cat# 1800 Oligonucleotides See Tables S6 and S7 for details N/A N/A Software and algorithms NIS-Elements AR Nikon RRID: SCR_014329 ZEN 3.1 Carl Zeiss RRID:SCR_013672 Image Lab Software Bio-rad RRID:SCR_014210 Fiji (ImageJ) Fiji (ImageJ) RRID: SCR_002285 Graphpad Prism 9 Graphpad Software RRID: SCR_002798 Biorender Biorender RRID:SCR_018361

    RNAscope:

    Article Title: Multi-chamber cardioids unravel human heart development and cardiac defects.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies TNNT2 Thermo Scientific Cat# MS-295-P TNNT2 Abcam Cat# ab45932; RRID: AB_956386 CDH5 (VE-Cadherin) Cell Signaling Technology Cat# 2500S PECAM1 (CD31) Agilent Technologies Cat# M082329-2 PECAM1 (CD31) R&D Systems Cat# AF806; RRID: AB_355617 HAND1 R&D Systems Cat# AF3168; RRID: AB_2115853 HAND2 Abcam Cat# ab200040; RRID: AB_2923502 NKX2-5 R&D Systems Cat# AF2444; RRID: AB_355269 IRX1 Thermo Scientific Cat# PA5-60261 TBX2 Novus Biologicals Cat# NBP1-89459 TBX3 R&D Systems Cat# AF4509; RRID: AB_2240328 E-Cadherin (CDH1) Cell Signaling Technology Europe Cat# 3195 N-Cadherin (CDH2) BD Biosciences Cat# 610920; RRID: AB_2077527 HEY2 Proteintech Cat# 10597-1-AP; RRID: AB_2118415 ISL1 DSHB Cat# 39.4D5; RRID: AB_2314683 FOXF1 R&D Systems Cat# AF4798-SP; RRID: AB_2105588 FOXC2 Bio-Techne Cat# AF5044-SP; RRID: AB_2105268 NR2F2 (COUP-TFII) R&D Systems Cat# PP-H7147-00; RRID: AB_2155627 MKI67 BD Biosciences Cat# 556003; RRID: AB_396287 TBX5 Sigma-Aldrich Cat# HPA008786; RRID: AB_10601720 NPPA Thermo Scientific Cat# PA5-63543 MYL2 Abcam Cat# ab79935; RRID: AB_1952220 Anti-a-Actinin antibody Sigma-Aldrich Cat# A7811; RRID: AB_476766 DAPI Sigma-Aldrich Cat# D9542 Hoechst33342 (EdU kit) Thermo Scientific Cat# C10640 Fluo-4 AM Thermo Scientific Cat# F14217 FluoVolt Thermo Scientific Cat# F10488 Donkey anti-Sheep IgG, Secondary Antibody, Alexa Fluor 647 Thermo Scientific Cat# A21448 Donkey anti-Mouse IgG, Secondary Antibody, Alexa Fluor 488 Thermo Scientific Cat# A21202 Donkey anti-Mouse IgG, Secondary Antibody, Alexa Fluor 594 Thermo Scientific Cat# A21203 Donkey anti-Mouse IgG, Secondary Antibody, Alexa Fluor 647 Thermo Scientific Cat# A31571 Donkey anti-Rabbit IgG, Secondary Antibody, Alexa Fluor 488 Thermo Scientific Cat# A21206 Donkey anti-Rabbit IgG, Secondary Antibody, Alexa Fluor 594 Thermo Scientific Cat# A21207 Donkey anti-Rabbit IgG, Secondary Antibody, Alexa Fluor 647 Thermo Scientific Cat# A31573 Donkey anti-Goat IgG, Secondary Antibody, Alexa Fluor 488 Thermo Scientific Cat# A11055 Donkey anti-Goat IgG, Secondary Antibody, Alexa Fluor 594 Thermo Scientific Cat# A11058 (Continued on next page) e1 Cell 186, 5587–5605.e1–e13, December 7, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Goat IgG, Secondary Antibody, Alexa Fluor 647 Thermo Scientific Cat# A21447 Chemicals, peptides, and recombinant proteins RNA-scope probe HOXB1 Bio-Techne Sales Corp. N/A RNA-scope probe TBX1 Bio-Techne Sales Corp. N/A HCR probe WNT5A Molecular Instruments N/A HCR probe IRX4 Molecular Instruments N/A HCR amplifier B3 (Alexa-546) Molecular Instruments N/A Antibiotic-Antimycotic Thermo Scientific #15240062 Y-27632 Tocris #1254 Vitronectin XF Stem Cell Technologies #7180 Laminin-511 E8 fragment AMSBIO #AMS.892 011 Fibronectin Sigma-Aldrich #F1141 Zebrafish FGF2 Cambridge University N/A hFGF2 QKine #Qk053 TGFb1 R&D Systems 240-B-010 LY294002 Tocris #1130 Activin A Cambridge University Activin A1 BMP4 R&D Systems 314-BP-050 CHIR99021 R&D Systems RD-4423/50 Insulin Roche #11376497001 VEGF165 Peprotech AF-100-20 IWP2 Tocris #3533 Retinoic Acid Sigma-Aldrich #R2625 SB431542 Tocris #1614 LDN-193189 Stemgent 04-0074 LY-411575 MedChemExpress HY-50752 Dexamethasone Sigma-Aldrich D4902 Indomethacin Sigma-Aldrich I7378 T3 hormone Sigma-Aldrich T6397 Chemically Defined Lipid Concentrate Thermo Scientific 11905031 PD0325901 Axon Med Chem #Axon1408 SB203580 R&D Systems #1202 PDGF-BB R&D Systems 220-BB-050 L-lactate Sigma-Aldrich #71718-10G Glucose Sigma-Aldrich #G7021-1KG Vitamin B12 Sigma-Aldrich #V6629-250MG Biotin Sigma-Aldrich #B4639-100MG Creatine monohydrate Sigma-Aldrich #C3630-100G Taurine Sigma-Aldrich #T0625-10G L-Carnitine Sigma-Aldrich #C0283-5G Non-Essential Amino Acids Solution Thermo Scientific #11140050 B-27 Supplement Thermo Scientific #17504044 KnockOut Serum Replacement Thermo Scientific #10828028 Thalidomide Sigma-Aldrich T144 Acitretin Sigma-Aldrich A0225000 Aspirin Tocris 4092 4-Aminopyridine Thermo Scientific A12405.18 (Continued on next page) ll OPEN ACCESS Cell 186, 5587–5605.e1–e13, December 7, 2023 e2 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER Perfluoro-n-octane sulfonate (PFOS) Thermo Scientific 16359117 Polystyrene latex microsphere, 0.05 micron Thermo Scientific 042711.AB SP-DiIC18(3) (Dil) Invitrogen D7777 DiIC18(5)-DS (DiD) Invitrogen D12730 Donkey Serum Bio-Rad Laboratories C06SB Isoprenaline Tocris 1747 Ivabradine Tocris 6542 Bay K 8644 Tocris 1546 16% Formaldehyde Thermo Scientific #28908 Sodium chloride (NaCl) Sigma-Aldrich S7653-1KG Potassium chloride (KCl) Sigma-Aldrich P9333-500G Calcium chloride dihydrate (CaCl2) Sigma-Aldrich C3881-500G Magnesium chloride hexahydrate (MgCl2) Sigma-Aldrich M2670-100G Sodium hydroxide solution (NaOH) Sigma-Aldrich 72068-100ML EGTA Sigma-Aldrich 03777-50G Adenosine 50-triphosphate magnesium salt (MgATP) Sigma-Aldrich A9187-1G Potassium hydroxide solution (KOH) Sigma-Aldrich 1.09108.1000 Hank’s Balanced Salt Solution (HBSS) Gibco #14175-053 TrypLE Express Gibco #12605010 DMEM/F12 with HEPES Gibco #11330032 Insulin-Transferrin-Selenium Gibco #41400045 L-Ascorbic Acid 2-phosphate Sigma-Aldrich A8960 Sodium Bicarbonate (7.5%) Gibco #25080094 F12 (with Glucose) Gibco #31765068 IMDM Gibco #21980065 DMEM with low glucose Sigma-Aldrich G8644 Monothioglycerol (MTG) Sigma-Aldrich M6145 Bovine Serum Albumin (BSA) Europa Bioproducts EQBAH-0500 PBS Gibco #14190094 STEMdiff cardiomyocyte dissociation kit Stem Cell Technologies #05025 Endothelial Cell Growth Medium MV PromoCell #PC-C-22020 DMEM no Glucose Gibco #11966025 Albumax Thermo Scientific #11020021 HEPES Sigma-Aldrich H4034-500G Cas9 2NLS Nuclease Synthego N/A Deposited data bulk RNA sequencing data GSE239891 Gene Expression Omnibus: SuperSeries GSE239891. single cell RNA sequencing data GSE239890 Gene Expression Omnibus: SuperSeries GSE239890.

    Affinity Magnetic Separation:

    Article Title: Multi-chamber cardioids unravel human heart development and cardiac defects.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies TNNT2 Thermo Scientific Cat# MS-295-P TNNT2 Abcam Cat# ab45932; RRID: AB_956386 CDH5 (VE-Cadherin) Cell Signaling Technology Cat# 2500S PECAM1 (CD31) Agilent Technologies Cat# M082329-2 PECAM1 (CD31) R&D Systems Cat# AF806; RRID: AB_355617 HAND1 R&D Systems Cat# AF3168; RRID: AB_2115853 HAND2 Abcam Cat# ab200040; RRID: AB_2923502 NKX2-5 R&D Systems Cat# AF2444; RRID: AB_355269 IRX1 Thermo Scientific Cat# PA5-60261 TBX2 Novus Biologicals Cat# NBP1-89459 TBX3 R&D Systems Cat# AF4509; RRID: AB_2240328 E-Cadherin (CDH1) Cell Signaling Technology Europe Cat# 3195 N-Cadherin (CDH2) BD Biosciences Cat# 610920; RRID: AB_2077527 HEY2 Proteintech Cat# 10597-1-AP; RRID: AB_2118415 ISL1 DSHB Cat# 39.4D5; RRID: AB_2314683 FOXF1 R&D Systems Cat# AF4798-SP; RRID: AB_2105588 FOXC2 Bio-Techne Cat# AF5044-SP; RRID: AB_2105268 NR2F2 (COUP-TFII) R&D Systems Cat# PP-H7147-00; RRID: AB_2155627 MKI67 BD Biosciences Cat# 556003; RRID: AB_396287 TBX5 Sigma-Aldrich Cat# HPA008786; RRID: AB_10601720 NPPA Thermo Scientific Cat# PA5-63543 MYL2 Abcam Cat# ab79935; RRID: AB_1952220 Anti-a-Actinin antibody Sigma-Aldrich Cat# A7811; RRID: AB_476766 DAPI Sigma-Aldrich Cat# D9542 Hoechst33342 (EdU kit) Thermo Scientific Cat# C10640 Fluo-4 AM Thermo Scientific Cat# F14217 FluoVolt Thermo Scientific Cat# F10488 Donkey anti-Sheep IgG, Secondary Antibody, Alexa Fluor 647 Thermo Scientific Cat# A21448 Donkey anti-Mouse IgG, Secondary Antibody, Alexa Fluor 488 Thermo Scientific Cat# A21202 Donkey anti-Mouse IgG, Secondary Antibody, Alexa Fluor 594 Thermo Scientific Cat# A21203 Donkey anti-Mouse IgG, Secondary Antibody, Alexa Fluor 647 Thermo Scientific Cat# A31571 Donkey anti-Rabbit IgG, Secondary Antibody, Alexa Fluor 488 Thermo Scientific Cat# A21206 Donkey anti-Rabbit IgG, Secondary Antibody, Alexa Fluor 594 Thermo Scientific Cat# A21207 Donkey anti-Rabbit IgG, Secondary Antibody, Alexa Fluor 647 Thermo Scientific Cat# A31573 Donkey anti-Goat IgG, Secondary Antibody, Alexa Fluor 488 Thermo Scientific Cat# A11055 Donkey anti-Goat IgG, Secondary Antibody, Alexa Fluor 594 Thermo Scientific Cat# A11058 (Continued on next page) e1 Cell 186, 5587–5605.e1–e13, December 7, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Goat IgG, Secondary Antibody, Alexa Fluor 647 Thermo Scientific Cat# A21447 Chemicals, peptides, and recombinant proteins RNA-scope probe HOXB1 Bio-Techne Sales Corp. N/A RNA-scope probe TBX1 Bio-Techne Sales Corp. N/A HCR probe WNT5A Molecular Instruments N/A HCR probe IRX4 Molecular Instruments N/A HCR amplifier B3 (Alexa-546) Molecular Instruments N/A Antibiotic-Antimycotic Thermo Scientific #15240062 Y-27632 Tocris #1254 Vitronectin XF Stem Cell Technologies #7180 Laminin-511 E8 fragment AMSBIO #AMS.892 011 Fibronectin Sigma-Aldrich #F1141 Zebrafish FGF2 Cambridge University N/A hFGF2 QKine #Qk053 TGFb1 R&D Systems 240-B-010 LY294002 Tocris #1130 Activin A Cambridge University Activin A1 BMP4 R&D Systems 314-BP-050 CHIR99021 R&D Systems RD-4423/50 Insulin Roche #11376497001 VEGF165 Peprotech AF-100-20 IWP2 Tocris #3533 Retinoic Acid Sigma-Aldrich #R2625 SB431542 Tocris #1614 LDN-193189 Stemgent 04-0074 LY-411575 MedChemExpress HY-50752 Dexamethasone Sigma-Aldrich D4902 Indomethacin Sigma-Aldrich I7378 T3 hormone Sigma-Aldrich T6397 Chemically Defined Lipid Concentrate Thermo Scientific 11905031 PD0325901 Axon Med Chem #Axon1408 SB203580 R&D Systems #1202 PDGF-BB R&D Systems 220-BB-050 L-lactate Sigma-Aldrich #71718-10G Glucose Sigma-Aldrich #G7021-1KG Vitamin B12 Sigma-Aldrich #V6629-250MG Biotin Sigma-Aldrich #B4639-100MG Creatine monohydrate Sigma-Aldrich #C3630-100G Taurine Sigma-Aldrich #T0625-10G L-Carnitine Sigma-Aldrich #C0283-5G Non-Essential Amino Acids Solution Thermo Scientific #11140050 B-27 Supplement Thermo Scientific #17504044 KnockOut Serum Replacement Thermo Scientific #10828028 Thalidomide Sigma-Aldrich T144 Acitretin Sigma-Aldrich A0225000 Aspirin Tocris 4092 4-Aminopyridine Thermo Scientific A12405.18 (Continued on next page) ll OPEN ACCESS Cell 186, 5587–5605.e1–e13, December 7, 2023 e2 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER Perfluoro-n-octane sulfonate (PFOS) Thermo Scientific 16359117 Polystyrene latex microsphere, 0.05 micron Thermo Scientific 042711.AB SP-DiIC18(3) (Dil) Invitrogen D7777 DiIC18(5)-DS (DiD) Invitrogen D12730 Donkey Serum Bio-Rad Laboratories C06SB Isoprenaline Tocris 1747 Ivabradine Tocris 6542 Bay K 8644 Tocris 1546 16% Formaldehyde Thermo Scientific #28908 Sodium chloride (NaCl) Sigma-Aldrich S7653-1KG Potassium chloride (KCl) Sigma-Aldrich P9333-500G Calcium chloride dihydrate (CaCl2) Sigma-Aldrich C3881-500G Magnesium chloride hexahydrate (MgCl2) Sigma-Aldrich M2670-100G Sodium hydroxide solution (NaOH) Sigma-Aldrich 72068-100ML EGTA Sigma-Aldrich 03777-50G Adenosine 50-triphosphate magnesium salt (MgATP) Sigma-Aldrich A9187-1G Potassium hydroxide solution (KOH) Sigma-Aldrich 1.09108.1000 Hank’s Balanced Salt Solution (HBSS) Gibco #14175-053 TrypLE Express Gibco #12605010 DMEM/F12 with HEPES Gibco #11330032 Insulin-Transferrin-Selenium Gibco #41400045 L-Ascorbic Acid 2-phosphate Sigma-Aldrich A8960 Sodium Bicarbonate (7.5%) Gibco #25080094 F12 (with Glucose) Gibco #31765068 IMDM Gibco #21980065 DMEM with low glucose Sigma-Aldrich G8644 Monothioglycerol (MTG) Sigma-Aldrich M6145 Bovine Serum Albumin (BSA) Europa Bioproducts EQBAH-0500 PBS Gibco #14190094 STEMdiff cardiomyocyte dissociation kit Stem Cell Technologies #05025 Endothelial Cell Growth Medium MV PromoCell #PC-C-22020 DMEM no Glucose Gibco #11966025 Albumax Thermo Scientific #11020021 HEPES Sigma-Aldrich H4034-500G Cas9 2NLS Nuclease Synthego N/A Deposited data bulk RNA sequencing data GSE239891 Gene Expression Omnibus: SuperSeries GSE239891. single cell RNA sequencing data GSE239890 Gene Expression Omnibus: SuperSeries GSE239890.

    Knock-Out:

    Article Title: Multi-chamber cardioids unravel human heart development and cardiac defects.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies TNNT2 Thermo Scientific Cat# MS-295-P TNNT2 Abcam Cat# ab45932; RRID: AB_956386 CDH5 (VE-Cadherin) Cell Signaling Technology Cat# 2500S PECAM1 (CD31) Agilent Technologies Cat# M082329-2 PECAM1 (CD31) R&D Systems Cat# AF806; RRID: AB_355617 HAND1 R&D Systems Cat# AF3168; RRID: AB_2115853 HAND2 Abcam Cat# ab200040; RRID: AB_2923502 NKX2-5 R&D Systems Cat# AF2444; RRID: AB_355269 IRX1 Thermo Scientific Cat# PA5-60261 TBX2 Novus Biologicals Cat# NBP1-89459 TBX3 R&D Systems Cat# AF4509; RRID: AB_2240328 E-Cadherin (CDH1) Cell Signaling Technology Europe Cat# 3195 N-Cadherin (CDH2) BD Biosciences Cat# 610920; RRID: AB_2077527 HEY2 Proteintech Cat# 10597-1-AP; RRID: AB_2118415 ISL1 DSHB Cat# 39.4D5; RRID: AB_2314683 FOXF1 R&D Systems Cat# AF4798-SP; RRID: AB_2105588 FOXC2 Bio-Techne Cat# AF5044-SP; RRID: AB_2105268 NR2F2 (COUP-TFII) R&D Systems Cat# PP-H7147-00; RRID: AB_2155627 MKI67 BD Biosciences Cat# 556003; RRID: AB_396287 TBX5 Sigma-Aldrich Cat# HPA008786; RRID: AB_10601720 NPPA Thermo Scientific Cat# PA5-63543 MYL2 Abcam Cat# ab79935; RRID: AB_1952220 Anti-a-Actinin antibody Sigma-Aldrich Cat# A7811; RRID: AB_476766 DAPI Sigma-Aldrich Cat# D9542 Hoechst33342 (EdU kit) Thermo Scientific Cat# C10640 Fluo-4 AM Thermo Scientific Cat# F14217 FluoVolt Thermo Scientific Cat# F10488 Donkey anti-Sheep IgG, Secondary Antibody, Alexa Fluor 647 Thermo Scientific Cat# A21448 Donkey anti-Mouse IgG, Secondary Antibody, Alexa Fluor 488 Thermo Scientific Cat# A21202 Donkey anti-Mouse IgG, Secondary Antibody, Alexa Fluor 594 Thermo Scientific Cat# A21203 Donkey anti-Mouse IgG, Secondary Antibody, Alexa Fluor 647 Thermo Scientific Cat# A31571 Donkey anti-Rabbit IgG, Secondary Antibody, Alexa Fluor 488 Thermo Scientific Cat# A21206 Donkey anti-Rabbit IgG, Secondary Antibody, Alexa Fluor 594 Thermo Scientific Cat# A21207 Donkey anti-Rabbit IgG, Secondary Antibody, Alexa Fluor 647 Thermo Scientific Cat# A31573 Donkey anti-Goat IgG, Secondary Antibody, Alexa Fluor 488 Thermo Scientific Cat# A11055 Donkey anti-Goat IgG, Secondary Antibody, Alexa Fluor 594 Thermo Scientific Cat# A11058 (Continued on next page) e1 Cell 186, 5587–5605.e1–e13, December 7, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Goat IgG, Secondary Antibody, Alexa Fluor 647 Thermo Scientific Cat# A21447 Chemicals, peptides, and recombinant proteins RNA-scope probe HOXB1 Bio-Techne Sales Corp. N/A RNA-scope probe TBX1 Bio-Techne Sales Corp. N/A HCR probe WNT5A Molecular Instruments N/A HCR probe IRX4 Molecular Instruments N/A HCR amplifier B3 (Alexa-546) Molecular Instruments N/A Antibiotic-Antimycotic Thermo Scientific #15240062 Y-27632 Tocris #1254 Vitronectin XF Stem Cell Technologies #7180 Laminin-511 E8 fragment AMSBIO #AMS.892 011 Fibronectin Sigma-Aldrich #F1141 Zebrafish FGF2 Cambridge University N/A hFGF2 QKine #Qk053 TGFb1 R&D Systems 240-B-010 LY294002 Tocris #1130 Activin A Cambridge University Activin A1 BMP4 R&D Systems 314-BP-050 CHIR99021 R&D Systems RD-4423/50 Insulin Roche #11376497001 VEGF165 Peprotech AF-100-20 IWP2 Tocris #3533 Retinoic Acid Sigma-Aldrich #R2625 SB431542 Tocris #1614 LDN-193189 Stemgent 04-0074 LY-411575 MedChemExpress HY-50752 Dexamethasone Sigma-Aldrich D4902 Indomethacin Sigma-Aldrich I7378 T3 hormone Sigma-Aldrich T6397 Chemically Defined Lipid Concentrate Thermo Scientific 11905031 PD0325901 Axon Med Chem #Axon1408 SB203580 R&D Systems #1202 PDGF-BB R&D Systems 220-BB-050 L-lactate Sigma-Aldrich #71718-10G Glucose Sigma-Aldrich #G7021-1KG Vitamin B12 Sigma-Aldrich #V6629-250MG Biotin Sigma-Aldrich #B4639-100MG Creatine monohydrate Sigma-Aldrich #C3630-100G Taurine Sigma-Aldrich #T0625-10G L-Carnitine Sigma-Aldrich #C0283-5G Non-Essential Amino Acids Solution Thermo Scientific #11140050 B-27 Supplement Thermo Scientific #17504044 KnockOut Serum Replacement Thermo Scientific #10828028 Thalidomide Sigma-Aldrich T144 Acitretin Sigma-Aldrich A0225000 Aspirin Tocris 4092 4-Aminopyridine Thermo Scientific A12405.18 (Continued on next page) ll OPEN ACCESS Cell 186, 5587–5605.e1–e13, December 7, 2023 e2 Resource .. REAGENT or RESOURCE SOURCE IDENTIFIER Perfluoro-n-octane sulfonate (PFOS) Thermo Scientific 16359117 Polystyrene latex microsphere, 0.05 micron Thermo Scientific 042711.AB SP-DiIC18(3) (Dil) Invitrogen D7777 DiIC18(5)-DS (DiD) Invitrogen D12730 Donkey Serum Bio-Rad Laboratories C06SB Isoprenaline Tocris 1747 Ivabradine Tocris 6542 Bay K 8644 Tocris 1546 16% Formaldehyde Thermo Scientific #28908 Sodium chloride (NaCl) Sigma-Aldrich S7653-1KG Potassium chloride (KCl) Sigma-Aldrich P9333-500G Calcium chloride dihydrate (CaCl2) Sigma-Aldrich C3881-500G Magnesium chloride hexahydrate (MgCl2) Sigma-Aldrich M2670-100G Sodium hydroxide solution (NaOH) Sigma-Aldrich 72068-100ML EGTA Sigma-Aldrich 03777-50G Adenosine 50-triphosphate magnesium salt (MgATP) Sigma-Aldrich A9187-1G Potassium hydroxide solution (KOH) Sigma-Aldrich 1.09108.1000 Hank’s Balanced Salt Solution (HBSS) Gibco #14175-053 TrypLE Express Gibco #12605010 DMEM/F12 with HEPES Gibco #11330032 Insulin-Transferrin-Selenium Gibco #41400045 L-Ascorbic Acid 2-phosphate Sigma-Aldrich A8960 Sodium Bicarbonate (7.5%) Gibco #25080094 F12 (with Glucose) Gibco #31765068 IMDM Gibco #21980065 DMEM with low glucose Sigma-Aldrich G8644 Monothioglycerol (MTG) Sigma-Aldrich M6145 Bovine Serum Albumin (BSA) Europa Bioproducts EQBAH-0500 PBS Gibco #14190094 STEMdiff cardiomyocyte dissociation kit Stem Cell Technologies #05025 Endothelial Cell Growth Medium MV PromoCell #PC-C-22020 DMEM no Glucose Gibco #11966025 Albumax Thermo Scientific #11020021 HEPES Sigma-Aldrich H4034-500G Cas9 2NLS Nuclease Synthego N/A Deposited data bulk RNA sequencing data GSE239891 Gene Expression Omnibus: SuperSeries GSE239891. single cell RNA sequencing data GSE239890 Gene Expression Omnibus: SuperSeries GSE239890.



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    ldn bmp inhibitor stemgent 04  (ReproCELL)
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    Ldn Bmp Inhibitor Stemgent 04, supplied by ReproCELL, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ldn bmp inhibitor stemgent 04 - by Bioz Stars, 2026-09
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